mouse hepatocyte cell line Search Results


90
DS Pharma Biomedical mouse hepatocyte cell line nmuli cells
Increased elimination of L. monocytogenes in PGLYRP-1-overexpressing hepatocytes. <t>NMuLi</t> <t>hepatocyte</t> cells seeded at a concentration of 2 × 105 cells/well were transfected with pEGFP-C2/PGLYRP-1 or pEGFP-C2 control vector. Both transfected cells were infected with L. monocytogenes for 30 min, and cell cultivation was continued with the elimination of extracellular bacteria by gentamicin. After 6 h of cultivation, L. monocytogenes cells were labeled with rhodamine, and rhodamine-labeled bacterial numbers in GFP-labeled cells were counted and the ratio of the bacterial number to the cell number was calculated. An asterisk indicates a significant difference from the pEGFP-C2-transfected group at P < 0.05.
Mouse Hepatocyte Cell Line Nmuli Cells, supplied by DS Pharma Biomedical, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mouse hepatocyte cell line nmuli cells - by Bioz Stars, 2026-02
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90
Korean Cell Line Bank mouse hepatocytes
Increased elimination of L. monocytogenes in PGLYRP-1-overexpressing hepatocytes. <t>NMuLi</t> <t>hepatocyte</t> cells seeded at a concentration of 2 × 105 cells/well were transfected with pEGFP-C2/PGLYRP-1 or pEGFP-C2 control vector. Both transfected cells were infected with L. monocytogenes for 30 min, and cell cultivation was continued with the elimination of extracellular bacteria by gentamicin. After 6 h of cultivation, L. monocytogenes cells were labeled with rhodamine, and rhodamine-labeled bacterial numbers in GFP-labeled cells were counted and the ratio of the bacterial number to the cell number was calculated. An asterisk indicates a significant difference from the pEGFP-C2-transfected group at P < 0.05.
Mouse Hepatocytes, supplied by Korean Cell Line Bank, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mouse hepatocytes - by Bioz Stars, 2026-02
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90
BioVector NTCC alm12 cell (mouse normal hepatocyte) line
Increased elimination of L. monocytogenes in PGLYRP-1-overexpressing hepatocytes. <t>NMuLi</t> <t>hepatocyte</t> cells seeded at a concentration of 2 × 105 cells/well were transfected with pEGFP-C2/PGLYRP-1 or pEGFP-C2 control vector. Both transfected cells were infected with L. monocytogenes for 30 min, and cell cultivation was continued with the elimination of extracellular bacteria by gentamicin. After 6 h of cultivation, L. monocytogenes cells were labeled with rhodamine, and rhodamine-labeled bacterial numbers in GFP-labeled cells were counted and the ratio of the bacterial number to the cell number was calculated. An asterisk indicates a significant difference from the pEGFP-C2-transfected group at P < 0.05.
Alm12 Cell (Mouse Normal Hepatocyte) Line, supplied by BioVector NTCC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
alm12 cell (mouse normal hepatocyte) line - by Bioz Stars, 2026-02
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90
Lonza mouse hepatocyte cell line aml12
A: Nitrite production after 24-h incubation of RAW 264.7 cells with <t>AML12</t> hepatocyte-derived DAMPs. Data are plotted as mean ± SEM with N = 7-8 per group. B: Real-time ROS production after 24-h incubation of RAW 264.7 cells with AML12-derived DAMPs. Data are plotted as mean ± SEM with N = 4 per group. * indicates p < 0.05, ** indicates p < 0.01, and *** indicates p < 0.001 compared to the medium group. Abbreviations: c, centrifuged; LPS, lipopolysaccharide; PMA, phorbol 12-myristate 13-acetate; DCF, dichlorofluorescein.
Mouse Hepatocyte Cell Line Aml12, supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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90
JCRB Cell Bank mouse hepatocyte cell line nctc1469
A: Nitrite production after 24-h incubation of RAW 264.7 cells with <t>AML12</t> hepatocyte-derived DAMPs. Data are plotted as mean ± SEM with N = 7-8 per group. B: Real-time ROS production after 24-h incubation of RAW 264.7 cells with AML12-derived DAMPs. Data are plotted as mean ± SEM with N = 4 per group. * indicates p < 0.05, ** indicates p < 0.01, and *** indicates p < 0.001 compared to the medium group. Abbreviations: c, centrifuged; LPS, lipopolysaccharide; PMA, phorbol 12-myristate 13-acetate; DCF, dichlorofluorescein.
Mouse Hepatocyte Cell Line Nctc1469, supplied by JCRB Cell Bank, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mouse hepatocyte cell line nctc1469/product/JCRB Cell Bank
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mouse hepatocyte cell line nctc1469 - by Bioz Stars, 2026-02
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90
Nissui Pharmaceutical mouse hepatocyte cell line nmuli cells
A: Nitrite production after 24-h incubation of RAW 264.7 cells with <t>AML12</t> hepatocyte-derived DAMPs. Data are plotted as mean ± SEM with N = 7-8 per group. B: Real-time ROS production after 24-h incubation of RAW 264.7 cells with AML12-derived DAMPs. Data are plotted as mean ± SEM with N = 4 per group. * indicates p < 0.05, ** indicates p < 0.01, and *** indicates p < 0.001 compared to the medium group. Abbreviations: c, centrifuged; LPS, lipopolysaccharide; PMA, phorbol 12-myristate 13-acetate; DCF, dichlorofluorescein.
Mouse Hepatocyte Cell Line Nmuli Cells, supplied by Nissui Pharmaceutical, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mouse hepatocyte cell line nmuli cells - by Bioz Stars, 2026-02
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Image Search Results


Increased elimination of L. monocytogenes in PGLYRP-1-overexpressing hepatocytes. NMuLi hepatocyte cells seeded at a concentration of 2 × 105 cells/well were transfected with pEGFP-C2/PGLYRP-1 or pEGFP-C2 control vector. Both transfected cells were infected with L. monocytogenes for 30 min, and cell cultivation was continued with the elimination of extracellular bacteria by gentamicin. After 6 h of cultivation, L. monocytogenes cells were labeled with rhodamine, and rhodamine-labeled bacterial numbers in GFP-labeled cells were counted and the ratio of the bacterial number to the cell number was calculated. An asterisk indicates a significant difference from the pEGFP-C2-transfected group at P < 0.05.

Journal: Infection and Immunity

Article Title: Mouse Peptidoglycan Recognition Protein PGLYRP-1 Plays a Role in the Host Innate Immune Response against Listeria monocytogenes Infection

doi: 10.1128/IAI.00466-10

Figure Lengend Snippet: Increased elimination of L. monocytogenes in PGLYRP-1-overexpressing hepatocytes. NMuLi hepatocyte cells seeded at a concentration of 2 × 105 cells/well were transfected with pEGFP-C2/PGLYRP-1 or pEGFP-C2 control vector. Both transfected cells were infected with L. monocytogenes for 30 min, and cell cultivation was continued with the elimination of extracellular bacteria by gentamicin. After 6 h of cultivation, L. monocytogenes cells were labeled with rhodamine, and rhodamine-labeled bacterial numbers in GFP-labeled cells were counted and the ratio of the bacterial number to the cell number was calculated. An asterisk indicates a significant difference from the pEGFP-C2-transfected group at P < 0.05.

Article Snippet: Mouse hepatocyte cell line NMuLi cells (DS Pharma Biomedical, Osaka, Japan) were maintained in DMEM with 10% (vol/vol) FCS.

Techniques: Concentration Assay, Transfection, Control, Plasmid Preparation, Infection, Bacteria, Labeling

A: Nitrite production after 24-h incubation of RAW 264.7 cells with AML12 hepatocyte-derived DAMPs. Data are plotted as mean ± SEM with N = 7-8 per group. B: Real-time ROS production after 24-h incubation of RAW 264.7 cells with AML12-derived DAMPs. Data are plotted as mean ± SEM with N = 4 per group. * indicates p < 0.05, ** indicates p < 0.01, and *** indicates p < 0.001 compared to the medium group. Abbreviations: c, centrifuged; LPS, lipopolysaccharide; PMA, phorbol 12-myristate 13-acetate; DCF, dichlorofluorescein.

Journal: Journal of Clinical and Translational Research

Article Title: IL-23 and IL-17A are not involved in hepatic/ischemia reperfusion injury in mouse and man

doi:

Figure Lengend Snippet: A: Nitrite production after 24-h incubation of RAW 264.7 cells with AML12 hepatocyte-derived DAMPs. Data are plotted as mean ± SEM with N = 7-8 per group. B: Real-time ROS production after 24-h incubation of RAW 264.7 cells with AML12-derived DAMPs. Data are plotted as mean ± SEM with N = 4 per group. * indicates p < 0.05, ** indicates p < 0.01, and *** indicates p < 0.001 compared to the medium group. Abbreviations: c, centrifuged; LPS, lipopolysaccharide; PMA, phorbol 12-myristate 13-acetate; DCF, dichlorofluorescein.

Article Snippet: The mouse hepatocyte cell line AML12 was cultured in William’s E (WE) medium (Lonza, Basel, Switzerland) supplemented with 10% (v/v) fetal bovine serum (FBS, Thermo Fisher Scientific, Waltham, MA), penicillin (100 U/mL, Thermo Fisher Scientific), streptomycin (100 μg/mL, Thermo Fisher Scientific), insulin (5 μg/mL, Sigma-Aldrich, St. Louis, MO), L-glutamine (2 mM, Thermo Fisher Scientific), and hydrocortisone hemisuccinate (50 μM, Sigma-Aldrich).

Techniques: Incubation, Derivative Assay

Pro-inflammatory signaling by DAMP-exposed RAW 264.7 macrophages. A: TNFα, B: IL-1β, and C: IL-6 mRNA expression after 1-h and 6-h DAMP incubation. All results are presented as fold upregulation compared to medium incubation (N = 3-4 per group). D-G: Luciferase reporter assay of RAW 264.7 NF-κB/LUCPorter cells following medium-, DAMP-, or LPS stimulation after D: 6 h, E: 12 h, and F: 24 h (N = 3 per group). G: Luciferase reporter assay after stimulation with DAMPs derived from ischemia-subjected necrotic cells, medium, or LPS after 24 h of exposure (N = 3 per group). Luciferase activity is expressed as the fold change relative to control. H: IL-23 production by murine macrophages in response to AML12 hepatocyte-derived DAMPs measured by ELISA in RAW 264.7 cell supernatant and corrected for protein (N = 4 per group). All data represent mean ± SEM. * indicates p < 0.05, ** indicates p < 0.01, *** indicates p < 0.001 compared to the medium samples.

Journal: Journal of Clinical and Translational Research

Article Title: IL-23 and IL-17A are not involved in hepatic/ischemia reperfusion injury in mouse and man

doi:

Figure Lengend Snippet: Pro-inflammatory signaling by DAMP-exposed RAW 264.7 macrophages. A: TNFα, B: IL-1β, and C: IL-6 mRNA expression after 1-h and 6-h DAMP incubation. All results are presented as fold upregulation compared to medium incubation (N = 3-4 per group). D-G: Luciferase reporter assay of RAW 264.7 NF-κB/LUCPorter cells following medium-, DAMP-, or LPS stimulation after D: 6 h, E: 12 h, and F: 24 h (N = 3 per group). G: Luciferase reporter assay after stimulation with DAMPs derived from ischemia-subjected necrotic cells, medium, or LPS after 24 h of exposure (N = 3 per group). Luciferase activity is expressed as the fold change relative to control. H: IL-23 production by murine macrophages in response to AML12 hepatocyte-derived DAMPs measured by ELISA in RAW 264.7 cell supernatant and corrected for protein (N = 4 per group). All data represent mean ± SEM. * indicates p < 0.05, ** indicates p < 0.01, *** indicates p < 0.001 compared to the medium samples.

Article Snippet: The mouse hepatocyte cell line AML12 was cultured in William’s E (WE) medium (Lonza, Basel, Switzerland) supplemented with 10% (v/v) fetal bovine serum (FBS, Thermo Fisher Scientific, Waltham, MA), penicillin (100 U/mL, Thermo Fisher Scientific), streptomycin (100 μg/mL, Thermo Fisher Scientific), insulin (5 μg/mL, Sigma-Aldrich, St. Louis, MO), L-glutamine (2 mM, Thermo Fisher Scientific), and hydrocortisone hemisuccinate (50 μM, Sigma-Aldrich).

Techniques: Expressing, Incubation, Luciferase, Reporter Assay, Derivative Assay, Activity Assay, Control, Enzyme-linked Immunosorbent Assay