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BioVector NTCC
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Image Search Results
Journal: Infection and Immunity
Article Title: Mouse Peptidoglycan Recognition Protein PGLYRP-1 Plays a Role in the Host Innate Immune Response against Listeria monocytogenes Infection
doi: 10.1128/IAI.00466-10
Figure Lengend Snippet: Increased elimination of L. monocytogenes in PGLYRP-1-overexpressing hepatocytes. NMuLi hepatocyte cells seeded at a concentration of 2 × 105 cells/well were transfected with pEGFP-C2/PGLYRP-1 or pEGFP-C2 control vector. Both transfected cells were infected with L. monocytogenes for 30 min, and cell cultivation was continued with the elimination of extracellular bacteria by gentamicin. After 6 h of cultivation, L. monocytogenes cells were labeled with rhodamine, and rhodamine-labeled bacterial numbers in GFP-labeled cells were counted and the ratio of the bacterial number to the cell number was calculated. An asterisk indicates a significant difference from the pEGFP-C2-transfected group at P < 0.05.
Article Snippet:
Techniques: Concentration Assay, Transfection, Control, Plasmid Preparation, Infection, Bacteria, Labeling
Journal: Journal of Clinical and Translational Research
Article Title: IL-23 and IL-17A are not involved in hepatic/ischemia reperfusion injury in mouse and man
doi:
Figure Lengend Snippet: A: Nitrite production after 24-h incubation of RAW 264.7 cells with AML12 hepatocyte-derived DAMPs. Data are plotted as mean ± SEM with N = 7-8 per group. B: Real-time ROS production after 24-h incubation of RAW 264.7 cells with AML12-derived DAMPs. Data are plotted as mean ± SEM with N = 4 per group. * indicates p < 0.05, ** indicates p < 0.01, and *** indicates p < 0.001 compared to the medium group. Abbreviations: c, centrifuged; LPS, lipopolysaccharide; PMA, phorbol 12-myristate 13-acetate; DCF, dichlorofluorescein.
Article Snippet: The
Techniques: Incubation, Derivative Assay
Journal: Journal of Clinical and Translational Research
Article Title: IL-23 and IL-17A are not involved in hepatic/ischemia reperfusion injury in mouse and man
doi:
Figure Lengend Snippet: Pro-inflammatory signaling by DAMP-exposed RAW 264.7 macrophages. A: TNFα, B: IL-1β, and C: IL-6 mRNA expression after 1-h and 6-h DAMP incubation. All results are presented as fold upregulation compared to medium incubation (N = 3-4 per group). D-G: Luciferase reporter assay of RAW 264.7 NF-κB/LUCPorter cells following medium-, DAMP-, or LPS stimulation after D: 6 h, E: 12 h, and F: 24 h (N = 3 per group). G: Luciferase reporter assay after stimulation with DAMPs derived from ischemia-subjected necrotic cells, medium, or LPS after 24 h of exposure (N = 3 per group). Luciferase activity is expressed as the fold change relative to control. H: IL-23 production by murine macrophages in response to AML12 hepatocyte-derived DAMPs measured by ELISA in RAW 264.7 cell supernatant and corrected for protein (N = 4 per group). All data represent mean ± SEM. * indicates p < 0.05, ** indicates p < 0.01, *** indicates p < 0.001 compared to the medium samples.
Article Snippet: The
Techniques: Expressing, Incubation, Luciferase, Reporter Assay, Derivative Assay, Activity Assay, Control, Enzyme-linked Immunosorbent Assay